Article
[Interaction (integration and excision) of the pRP19.6 plasmid, a derivative of the RP1 plasmid containing duplicated sequence IS21, with the chromosome of Escherichia coli K12].
Molekuliarnaia biologiia - 1 Jan 2000
Danilevich V N, Kostiuchenko D A, Negriĭ N V
Abstract excerpt
A pRP19.6 plasmid is the derivative of the temperature sensitive RPlts12 plasmid and contains a duplicated IS21 (IS8) element. Using temperature sensitive pRP19.6 replication, Hfr strains have been obtained by integration of the plasmid into the chromosome of E. coli rec+ and recA- cells and thei...
Topics
- Chromosome Deletion
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Restriction Enzymes
- Drug Resistance, Microbial
- Escherichia coli
- Mutation
- Plasmids
- Recombination, Genetic