Article
[Dissociation of unstable cointegrate plasmids formed during transposition of IS1 element: the role of IS1 encoded recombinase].
Molekuliarnaia biologiia - 1 Jan 2000
Danilevich V N
Abstract excerpt
The properties of IS1/Tn9'-mediated cointegrates between plasmids pDK57 (pBR322:: :: Tn9') and pRP3.1--the deletion derivative of RP1 were investigated. It was found that IS1/Tn9'-mediated integration of pDK57 into the active transcribed regions of pRP3.1 (in particular kan and tet genes) leads to formation of unstable cointegrates capable of resolving in E. coli K-12 rec+ and recA cells. The structure of...
Topics
- Conjugation, Genetic
- DNA Nucleotidyltransferases
- DNA Transposable Elements
- Escherichia coli
- Models, Genetic
- Molecular Weight
- Mutation
- Plasmids
- Recombination, Genetic
