Article
[Isolation and functional-structural characteristics of Bacillus polymyxa RP4::Mucts62 transcipients].
Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jan 2001
Il'iashenko B N
Abstract excerpt
Conjugative-like transfer of hybrid plasmid RP4::Mucts62 from Escherichia coli to plasmid-free Bacillus polymyxa was carried out. Bacillus transcipients are detected by the markers of kanamycin and tetracyclin resistance RP4 and thermal sensitivity to growth at 40-42 degrees C, determined by prophage Mucts62 in the plasmid. The technique of transception using millipore filters on solid media has been improved....
Topics
- Bacillus
- Bacillus Phages
- Conjugation, Genetic
- Escherichia coli
- Kanamycin Resistance
- Phenotype
- Plasmids
- Restriction Mapping
- Sequence Deletion
- Tetracycline Resistance