Article
[Study of the role of the insA open reading frame in the IS1 insertion element structure during transposition and resolution of the IS1 mediated cointegrates].
Molekuliarnaia biologiia - 1 Jan 2000
Danilevich V N, Duzhiĭ D E, Veĭko V P, Ratmanova K I, Shekhter I I, Sukhodolets V V
Abstract excerpt
In order to elucidate the function of the IS1 insA gene derivatives of plasmid pUC19::Tn9' with insertions of synthetic oligonucleotides were obtained. The latter are equal or multiple of 9 b.p. in length and are located in the Pst1 site within each of the two IS1 copies of the Tn9' transposon. The insertions of the nine base oligonucleotides code for the neutral amino acids and do not shift the reading frame....
Topics
- Base Sequence
- DNA Transposable Elements
- DNA, Bacterial
- Electrophoresis, Agar Gel
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Site-Directed