Article
Generation and Phenotypic Characterization of a CRISPR/Cas9-Engineered Cracd-Deficient Mouse Model for Post-Myocardial Infarction Remodeling Studies.
Journal of visualized experiments : JoVE - 8 Sept 2026
Zheng Shuang, Li Xiang, Li Fei, Ren Xuecong, Chen Jianxin, Zou Yunzeng, Yang Feng Hua, Xia Yu, Tan Weijiang
Abstract excerpt
Myocardial infarction (MI) remains a major cause of morbidity and mortality worldwide. This protocol describes a method for generating and characterizing a Cracd-deficient mouse line on the C57BL/6N background using CRISPR/Cas9 technology. Zygotes were co-injected with Cas9 mRNA, a gRNA construct, and a donor template designed to generate a 3153-bp genomic deletion. The edited allele was confirmed by PCR...
Read the complete abstract on PubMedTopics
- Animals
- Mice
- Myocardial Infarction
- Disease Models, Animal
- Mice, Inbred C57BL
- CRISPR-Cas Systems