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Article

Generation of full-length wild-type and mutated futsch transgenes in Drosophila-efficient Gibson Assembly of ultra-large cDNAs.

Fly - 1 Dec 2026

Wu Chunlai

Abstract excerpt

Drosophila Futsch is a key microtubule-associated protein (fly homolog of MAP1B) that regulates microtubule organization, synaptic terminal growth, and neuronal development. Functional analysis of futsch has long been limited by the inability to clone and express a full-length futsch transgene, owing to its exceptional size (~16.5 kb) and extensive repetitive sequences. Here, I present an efficient and...

Read the complete abstract on PubMed

Topics

Animals
Drosophila Proteins
DNA, Complementary
Transgenes
Cloning, Molecular
Microtubule-Associated Proteins
Mutation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Animals, Genetically Modified

Drosophila

Drosophila melanogaster

Source

Open in PubMed Open full text

Identifiers

PMID: 42697839
DOI: 10.1080/19336934.2026.2719251
PMCID: PMC13556936

Genes

MAP1B

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 25 Oct 2026.

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