Budding yeast Bud3 and Bud4 cooperatively organize septin architecture and support cytokinetic spatial memory.

Abstract excerpt

During cytokinesis, budding yeast assemble a double septin ring composed of circumferential filaments that compartmentalize the membrane at the bud neck. This structure's integrity depends on two proteins, Bud3 and Bud4. Using in vitro reconstitution assays, we demonstrate that Bud3 and Bud4 organize septin filaments in distinct ways and cooperate to assemble complex, higher order networks. They bind to different...

Topics

• Septins
• Cytokinesis
• Saccharomyces cerevisiae Proteins
• Saccharomyces cerevisiae
• Cell Cycle Proteins
• Protein Binding