The promise of long-read RNA-seq: reducing bias in analyses of allele imbalance.

Abstract excerpt

Inaccurate allele and gene expression counts due to map bias and genome ambiguity lead to high false positive and false negative rates in studies of allelic imbalance. We demonstrate that long read RNA sequencing (RNA-seq) and straightforward quality control measures can be used to reduce bias in allele counts in case studies from four species: Drosophila melanogaster, a diploid insect; Solanum tuberosum, an...

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