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Article

Performance of mechanically sheared DNA in multiplexed Oxford Nanopore sequencing for Salmonella Typhi genomic surveillance.

Microbial genomics - 1 Jul 2026

Maung Hsu Thinzar, Morita Masamoto, Han Su Myat, Mishima Hiroyuki, Koga Chisa, Evangelista Jan Wendzl C, Girasol Mark John, Suzuki Shuichi, Yoshiura Koh-Ichiro, Kodama Toshio, Smith Chris, Hiyoshi Hirotaka

Abstract excerpt

Pathogen genomic surveillance is essential for monitoring high-risk lineages and antimicrobial resistance in Salmonella enterica serovar Typhi (S. Typhi), yet implementing whole-genome sequencing in sentinel hospital laboratories remains challenging. We evaluated whether mechanically sheared DNA, paired with high accuracy or super accuracy (SUP) basecalling, enables reliable Oxford Nanopore Technologies...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 42507597
DOI: 10.1099/mgen.0.001805
PMCID: PMC13405487

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