Article
Evaluation of Prime Editing Efficiency in Human Immortalized MSC-TERT Cells with Osteogenic Potential for Modeling FGFR2-Linked Craniosynostosis.
The CRISPR journal - 1 Aug 2026
Gijsbertsen Max, Duarte Filipa M, Fuentes Manjón Ana, Maduro Tiany, Kassem Moustapha, van der Oost John, Mathijssen Irene M J, van Leeuwen Johannes P T M, van de Peppel Jeroen
Abstract excerpt
Craniosynostosis is a rare congenital bone condition where skull sutures fuse prematurely and is linked to mutations in over 60 genes. Generating mutation-specific in vitro models allows investigation of craniosynostosis-associated mutations without the need for patient-derived material or transgenic gene expression. Here, we developed a human in vitro disease model with the CRISPR-Cas9 prime editing variant,...
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