Engineering of protease-resistant phytase from Penicillium sp.: high thermal stability, low optimal temperature and pH.

Abstract excerpt

Phytase is widely used as a feed additive in industry. It is important to investigate the thermal stability, optimal pH and temperature and protease resistance of phytases in application. We introduced random mutations in a protease-resistance phytase gene of Penicillium sp. using Mn²+-dITP random mutation method, and identified two mutants 2-28 (T11A, G56E, L65F, Q144H and L151S) and 2-249 (T11A, H37Y, G56E,...

Topics

• 6-Phytase
• Animal Feed
• Catalysis
• Enzyme Stability
• Hot Temperature
• Hydrogen-Ion Concentration