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Clostridium difficile Genome Editing Using pyrE Alleles.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016

Ehsaan Muhammad, Kuehne Sarah A, Minton Nigel P

Abstract excerpt

Precise manipulation (in-frame deletions and substitutions) of the Clostridium difficile genome is possible through a two-stage process of single-crossover integration and subsequent isolation of double-crossover excision events using replication-defective plasmids that carry a counterselection marker. Use of a codA (cytosine deaminase) or pyrE (orotate phosphoribosyltransferase) as counter selection markers...

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PMID: 27507332
DOI: 10.1007/978-1-4939-6361-4_4

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ACE

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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