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Article

Novel activity of UDP-galactose-4-epimerase for free monosaccharide and activity improvement by active site-saturation mutagenesis.

Applied biochemistry and biotechnology - 1 Feb 2011

Kim Hye-Jung, Kang Sueng Yeun, Park Jong Jin, Kim Pil

Abstract excerpt

Uridine diphosphogalactose-4-epimerase (UDP-galactose-4-epimerase, GalE, EC 5.1.3.2) mediates the 4-epimerization of nucleic acid-activated galactose into UDP-glucose. To date, no enzyme is known to mediate 4-epimerization of free monosaccharide substrates. To determine the potential activity of GalE for free monosaccharide, Escherichia coli GalE was expressed and purified using Ni-affinity chromatography, and...

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PMID: 20717852
DOI: 10.1007/s12010-010-9052-7

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