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Article

Quantitative Assessment of Analytical Ambiguity in Reverse Sequence-Specific Oligonucleotide (rSSO)-Based HLA Typing Platforms.

International journal of immunogenetics - 1 Jun 2026

Yuan Fenghua, Wang Ning, Yuan Chao, Zhao Yan, Wang Xiaoyan

Abstract excerpt

Analytical ambiguity is an inherent and expected feature of reverse sequence-specific oligonucleotide (rSSO)-based HLA genotyping and reflects the size of the candidate allele space compatible with a given probe pattern at the analytical interpretation level. However, ambiguity is often treated as a binary attribute, which may obscure differences in its magnitude across platforms. We conducted a between-subject...

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PMID: 41983543
DOI: 10.1111/iji.70051

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