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Article

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing.

Nature communications - 19 Mar 2021

Ooi Kean Hean, Liu Mengying Mandy, Tay Jie Wen Douglas, Teo Seok Yee, Kaewsapsak Pornchai, Jin Shengyang, Lee Chun Kiat, Hou Jingwen, Maurer-Stroh Sebastian, Lin Weisi, Yan Benedict, Yan Gabriel, Gao Yong-Gui, Tan Meng How

Abstract excerpt

Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is robust to viral genome mutations and temperature, produces results fast, can be applied directly on nasopharyngeal (NP) specimens without RNA purification, and incorporates a human internal control within the same reaction. Specifically, we...

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Identifiers

PMID: 33741959
DOI: 10.1038/s41467-021-21996-6
PMCID: PMC7979722

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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