Characteristics of p53 and Smad4 immunohistochemistry in pancreatic ductal adenocarcinoma and validation by next-generation sequencing.

Abstract excerpt

BACKGROUND: Mutations in four major driver genes -KRAS, CDKN2A, TP53, and SMAD4- are central to the pathogenesis of pancreatic ductal adenocarcinoma (PDAC) and critically inform diagnosis, therapeutic decision-making, and prognostic assessment. Although next-generation sequencing (NGS) is widely regarded as the gold standard for detecting these mutations, its clinical application is often limited by suboptimal...

Topics

• Humans
• Smad4 Protein
• Carcinoma, Pancreatic Ductal
• Pancreatic Neoplasms
• Immunohistochemistry
• High-Throughput Nucleotide Sequencing