Construction of Corynebacterium glutamicum for efficient secretion and production of recombinant proteins using the technology of ARTP mutagenesis and fluorescen activated droplet microfluidic screening.

Abstract excerpt

In order to increase the secretion and expression of Corynebacterium glutamicum (C. glutamicum) as microbial cell factory, Actinobacillus porcine pleuropneumoniae outer membrane protein 7 (Omp7) was used as the target protein and a total of four mutant strains with efficient secretion and expression including CT1-109, CT2-9, CT3-28 and CT4-42 were obtained by the four rounds of Atmospheric and Room Temperature...

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