A tunable heterologous phosphatidylcholine biosynthetic system reveals distinct phosphatidylcholine requirements for strong and weak acid responses in Acetobacter pasteurianus.

Abstract excerpt

Acetic acid bacteria, including the genera Acetobacter and Komagataeibacter, utilize phosphatidylcholine (PC) as a major membrane component, which plays an important role in their membrane physiology. However, the significance of PC abundance within the membrane remains speculative. Here, we constructed a mutant strain of A. pasteurianus through genome modification, enabling choline-dependent PC production. This...

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