3-Keto-5α-steroid Δ1-dehydrogenase from <i>Rhodococcus erythropolis</i> SQ1 and its orthologue in <i>Mycobacterium tuberculosis</i> H37Rv are highly specific enzymes that function in cholesterol catabolism

Abstract excerpt

The Rhodococcus erythropolis SQ1 kstD3 gene was cloned, heterologously expressed and biochemically characterized as a KSTD3 (3-keto-5alpha-steroid Delta(1)-dehydrogenase). Upstream of kstD3, an ORF (open reading frame) with similarity to Delta(4) KSTD (3-keto-5alpha-steroid Delta(4)-dehydrogenase) was found, tentatively designated kst4D. Biochemical analysis revealed that the Delta(1) KSTD3 has a clear preference...

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