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Article

Cell foundry with high product specificity and catalytic activity for 21-deoxycortisol biotransformation.

Microbial cell factories - 13 Jun 2017

Xiong Shuting, Wang Ying, Yao Mingdong, Liu Hong, Zhou Xiao, Xiao Wenhai, Yuan Yingjin

Abstract excerpt

BACKGROUND: 21-deoxycortisol (21-DF) is the key intermediate to manufacture pharmaceutical glucocorticoids. Recently, a Japan patent has realized 21-DF production via biotransformation of 17-hydroxyprogesterone (17-OHP) by purified steroid 11β-hydroxylase CYP11B1. Due to the less costs on enzyme isolation, purification and stabilization as well as cofactors supply, whole-cell should be preferentially employed as...

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Biocatalysis
Biotransformation
Cattle
Ferredoxin-NADP Reductase
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Steroid 11-beta-Hydroxylase

Substrate Specificity

Synthetic Biology

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PMID: 28610588
DOI: 10.1186/s12934-017-0720-y
PMCID: PMC5470312

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