Solid-state nanopore sensing reveals conformational changes induced by a mutation in a neuron-specific tRNAArg.

Abstract excerpt

We demonstrate that solid-state nanopore sensing is a powerful single-molecule method for analyzing RNA conformational ensembles. As a model, we employed n-Tr20, a neuron-specific cytoplasmic tRNA$_{\mathrm{UCU}}^{\mathrm{Arg}}$, whose C50U mutation is associated with neurodegeneration in C57BL/6J mice. Maturation of the n-Tr20$^{\mathrm{C50U}}$ precursor is impaired as the mutation stabilizes a conformational...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cryoelectron Microscopy
• Share to Mice