Article
eIF3g binding to GUCG boxes located in mRNA coding regions enhances translation of mild heat shock response genes in the yeast Saccharomyces cerevisiae.
Nucleic acids research - 14 Jan 2026
Kato Hiroaki, Oguro Akihiro, Mao Yuanhui, Ochsner Kinsley, Casey Hailey, Sakai Grace, Wan Ji, Usuki Shingo, Tang Leiming, Asano Mizuki, Bardiya Nirmala, Kaufman Kade, Ishiguro Susumu, Tani Naoki, Kumagai Kazuyuki, Nakamura Akira, Singh Chingakham Ranjit, Sakamoto Taiichi, Obayashi Eiji, Qian Shu-Bing, Asano Katsura
Abstract excerpt
Eukaryotic translation initiation factor 3 (eIF3) is a multi-subunit complex that promotes ribosome recruitment and messenger RNA (mRNA) selection. Here, we show that its eIF3g subunit, along with the binding partner eIF3i, mediates transcript-specific translation under mild heat stress through direct RNA binding. First, SELEX experiments identified a short GUCG-centered motif preferentially recognized by eIF3g,...
Topics
- Saccharomyces cerevisiae
- Eukaryotic Initiation Factor-3
- Heat-Shock Response
- RNA, Messenger
- Protein Biosynthesis
- Saccharomyces cerevisiae Proteins
- Open Reading Frames
- Nucleotide Motifs