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Structural and functional characterization of the New Delhi metallo beta-lactamase-1 (NDM-1) D254A mutant: a biochemical, microbiological and bioinformatics study.

World journal of microbiology & biotechnology - 31 Dec 2025

Ali Abid, Fatima Kulsum, Farhat Nabeela, Khan Asad U

Abstract excerpt

New Delhi Metallo-β-lactamase-1 (NDM-1) belongs to the carbapenemase family of enzymes, which can hydrolyse a wide range of β-lactam antibiotics. To date, 91 NDM-1 variants have been reported globally. In this study, we generated a novel mutant, NDM-1 D254A, through site-directed mutagenesis to investigate the functional role of non-active site residue Asp254. The wild-type NDM-1 and D254A mutant were cloned,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 41474476
DOI: 10.1007/s11274-025-04766-0

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