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Repeated artificial mutagenesis of Bradyrhizobium diazoefficiens by gamma irradiation accelerates the acquisition of high-temperature tolerance.

Mutation research - 1 Jan 2025

Hase Yoshihiro, Nagafune Ikuko, Satoh Katsuya

Abstract excerpt

Effective mutant screening is critical for improving industrial microorganisms. This study was conducted to evaluate the effect of repeated mutagenesis with gamma rays on the experimental evolution of rhizobial high-temperature tolerance. Wild-type Bradyrhizobium diazoefficiens USDA110 cells grow optimally at 32-34 °C, but their growth is markedly retarded at 36 °C. Wild-type cells were subcultured in a 96-well...

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PMID: 41290508
DOI: 10.1016/j.mrfmmm.2025.111919

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