Article
Molecular characterization of microbial mutations induced by ion beam irradiation.
Mutation research - 1 Mar 2008
Ichida Hiroyuki, Matsuyama Tomoki, Ryuto Hiromichi, Hayashi Yoriko, Fukunishi Nobuhisa, Abe Tomoko, Koba Takato
Abstract excerpt
A positive selection system for gene disruption using a sucrose-sensitive transgenic rhizobium was established and used for the molecular characterization of mutations induced by ion beam irradiations. Single nucleotide substitutions, insertions, and deletions were found to occur in the sucrose sensitivity gene, sacB, when the reporter line was irradiated with highly accelerated carbon and iron ion beams. In all...
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