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High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples.

Journal of visualized experiments : JoVE - 31 Oct 2025

Stierli Salome, Sturzenegger Flurin, Jordaan Whitney Shannon, Micheli Sofia, Martins Joana Raquel, Sommer Lukas

Abstract excerpt

Skin injuries initiate a complex regenerative cascade that engages various cell types. Amongst those, peripheral glial cells play a critical role in ensuring the success of the repair process. However, our understanding of these processes remains incomplete due to limitations in current technologies. Therefore, the iterative-bleaching-extends-multiplexity (IBEX) method was employed to characterize changes to the...

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PMID: 41248009
DOI: 10.3791/67696

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