Article
Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping.
Scientific reports - 8 Aug 2016
Sung Kevin, Ding Yichen, Ma Jianguo, Chen Harrison, Huang Vincent, Cheng Michelle, Yang Cindy F, Kim Jocelyn T, Eguchi Daniel, Di Carlo Dino, Hsiai Tzung K, Nakano Atsushi, Kulkarni Rajan P
Abstract excerpt
Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging modalities. Here we describe the simplified CLARITY method (SCM) for tissue clearing that preserves epitopes of interest. We imaged the resulting tissues using light...
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