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Enhancement of Activity of Thermophilic Inorganic Pyrophosphatase Ton1914 via Site-Directed Mutagenesis.

Biomolecules - 30 Sept 2025

Liu Siyao, Yang Xinrui, Gao Renjun, Xie Guiqiu

Abstract excerpt

Inorganic pyrophosphatase (PPase) is an enzyme that catalyzes the hydrolysis of pyrophosphate (PPi) into two phosphates. Ton1914, a thermophilic inorganic pyrophosphatase derived from Thermococcus onnurineus NA1, has good thermal stability and an extremely high optimum temperature and has been shown to reduce pyrophosphate inhibition. In this study, eight sites were selected based on sequence alignment and...

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PMID: 41154624
DOI: 10.3390/biom15101395
PMCID: PMC12562645

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