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Article

Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX.

Applied and environmental microbiology - 15 Aug 2018

Zhou Wei, Huang Rui, Zhu Zhiguang, Zhang Yi-Heng P Job

Abstract excerpt

Thermostability and specific activity of enzymes are two of the most important properties for industrial biocatalysts. Here, we developed a petri dish-based double-layer high-throughput screening (HTS) strategy for rapid identification of desired mutants of polyphosphate glucokinase (PPGK) from a thermophilic actinobacterium, Thermobifida fusca YX, with both enhanced thermostability and activity. Escherichia coli...

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Identifiers

PMID: 29884753
DOI: 10.1128/AEM.01224-18
PMCID: PMC6070756

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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