Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Structure of a His170Tyr mutant of thermostable pNPPase from Geobacillus stearothermophilus.

Acta crystallographica. Section F, Structural biology communications - 1 Jun 2014

Shen Tiantian, Guo Zheng, Ji Chaoneng

Abstract excerpt

Using directed evolution based on random mutagenesis and heat-treated selection, a thermostable His170Tyr mutant of Geobacillus stearothermophilus thermostable p-nitrophenylphosphatase (TpNPPase) was obtained. The temperature at which the His170Tyr mutant lost 50% of its activity (T1/2) was found to be 4.40 K higher than that of wild-type TpNPPase, and the melting temperature of the His170Tyr mutant increased by...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Geobacillus stearothermophilus
Share to Histidine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Tyrosine

Source

Open in PubMed Open full text

Identifiers

PMID: 24915075
DOI: 10.1107/S2053230X14007341
PMCID: PMC4051519

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Structure of a His170Tyr mutant of thermostable pNPPase from Geobacillus stearothermophilus.PMID 24915075Read ↗

High-temperature behavior of hyperthermostable Thermotoga maritima xylanase XYN10B after designed and evolved mutations.Semantic proximity Read ↗

Effect of mutation of the conservative glycine residues Gly100 and Gly147 on stability of Escherichia coli inorganic pyrophosphatase.Semantic proximity Read ↗

Enhancement of Activity of Thermophilic Inorganic Pyrophosphatase Ton1914 via Site-Directed Mutagenesis.Semantic proximity Read ↗

Stabilization of the neutral protease of Bacillus stearothermophilus by removal of a buried water molecule.Semantic proximity Read ↗

Effect of the Lys62Ala Mutation on the Thermal Stability of BstHPr Protein by Molecular Dynamics.Semantic proximity Read ↗

Mutational effects on thermostable superoxide dismutase from Aquifex pyrophilus: understanding the molecular basis of protein thermostability.Semantic proximity Read ↗

Improved thermostability of an acidic xylanase from Aspergillus sulphureus by combined disulphide bridge introduction and proline residue substitution.Semantic proximity Read ↗

Divergence of structure and function in the haloacid dehalogenase enzyme superfamily: Bacteroides thetaiotaomicron BT2127 is an inorganic pyrophosphatase.Semantic proximity Read ↗

Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii, a thermoacidophilic archaeon.Semantic proximity Read ↗

Structural insights into methanol-stable variants of lipase T6 from Geobacillus stearothermophilus.Semantic proximity Read ↗

Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.Semantic proximity Read ↗

A ligand-induced conformational change in the Yersinia protein tyrosine phosphatase.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.