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DNAzyme-Driven Dynamic Cycling Biosensor with Dynamic Fluorescent and Cumulative Electrochemiluminescence Monitoring of Single-Nucleotide Variants.

Analytical chemistry - 7 Oct 2025

Yi Sirui, Chen Huiping, Qiu Haiyan, Lai Tingting, Li Xia, Wei Yuhuan, Kong Fenying, Hu Shanwen

Abstract excerpt

Nucleic acid-based constitutional dynamic networks (CDNs) can mimic biological events while revealing complex biological processes and their functionalities. In this study, we present a novel dual-signal biosensor that leverages a dissipative DNAzyme-driven network for the analysis of mutant targets. A dissipative cycle response to target in single-base resolution was designed based on a DNAzyme-driven network to...

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PMID: 40994158
DOI: 10.1021/acs.analchem.5c04377

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