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Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering.

Trends in biotechnology - 1 Jan 2026

Emelianov Georgii, Song Dong-Uk, Kamath Aporva, Kim Hyeongseop, Lee Geunyeong, Kim Ha-Neul, Kwon Kil Koang, Sung Bong Hyun, Lee Dae-Hee, Hillson Nathan J, Kim Haseong, Kim Sanguk, Lee Hyewon, Lee Seung-Goo

Abstract excerpt

Biofoundries serve as transformative platforms for accelerating the engineering of enzymes and microorganisms toward biomanufacturing. In this study, we developed scalable enzyme engineering workflows tailored for biofoundry applications, focusing on isoprene synthase (IspS) - a critical rate-limiting enzyme in the isoprene biosynthesis. By integrating computational mutation design based on sequence coevolution...

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PMID: 40946078
DOI: 10.1016/j.tibtech.2025.08.007

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