Improvement in the potency of a N1-methylpseudouridine-modified self-amplifying RNA through mutations in the RNA-dependent RNA polymerase.

Abstract excerpt

mRNA technology has widely incorporated modified nucleotides to temper innate immune activation by foreign RNA and achieve high levels of heterologous protein expression. However, the incorporation of modified nucleotides has had limited application to self-amplifying (sa) RNAs, as it impeded heterologous protein expression. Here, we used a reporter replicon of the attenuated TC-83 strain of Venezuelan equine...

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