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Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting

2 Jul 2025

Abstract excerpt

tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. Here, we present an assay for analyzing N 2 , N 2 -dimethylguanosine (m 2 2 G) modifications on tRNAs using an antibody-based detection approach. We describe steps for isolating tRNAs from human cells via ion-exchange chromatography, capturing specific tRNA iso-decoders on streptavidin magnetic beads, and detecting...

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PMID: 40601427
DOI: 10.1016/j.xpro.2025.103924

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