Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display

13 Feb 2004

Abstract excerpt

Homologous recombination in yeast can be exploited to reliably generate libraries of >10(7) transformants from a pool of PCR products and a linearized plasmid vector. Homology in the PCR insertion products drives shuffling of these genes in vivo by yeast homologous recombination. Two scFvs that share 89.8% homology were shuffled in vivo by homologous recombination, and chimeric genes were generated regardless of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Monoclonal and Polyclonal Antibodies Research
Share to Transgenic Plants and Applications
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 14978223
DOI: 10.1093/nar/gnh030

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Shuffled antibody libraries created by in vivo homologous recombination and yeast surface displayPMID 14978223Read ↗

Generating combinatorial diversity via engineered V(D)J-like recombination in Saccharomyces cerevisiaeSemantic proximity Read ↗

Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments.Semantic proximity Read ↗

Construction of a Large Synthetic Human Fab Antibody Library on Yeast Cell Surface by Optimized Yeast MatingSemantic proximity Read ↗

Engineering of Immunoglobulin Fc Heterodimers Using Yeast Surface-Displayed Combinatorial Fc Library Screening.Semantic proximity Read ↗

Isolating and engineering human antibodies using yeast surface displaySemantic proximity Read ↗

Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian CellsSemantic proximity Read ↗

Integrated mimicry of B cell antibody mutagenesis using yeast homologous recombination.Semantic proximity Read ↗

Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains.Semantic proximity Read ↗

Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast matingSemantic proximity Read ↗

Facile generation of antibody heavy and light chain diversities for yeast surface display by Golden Gate CloningSemantic proximity Read ↗

Engineering antibodies by yeast displaySemantic proximity Read ↗

An improved yeast transformation method for the generation of very large human antibody librariesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.