Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype.

doi:10.1038/317813a0

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Article

Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype.

Nature - 1 Jan 2000

Buller R M, Smith G L, Cremer K, Notkins A L, Moss B

Abstract excerpt

Recent advances in molecular genetics have led to the possibility of using large DNA viruses, such as vaccinia virus, as a biological delivery system for immunizing man against unrelated disease-causing agents. When live vaccinia virus recombinants expressing the hepatitis B virus surface antigen (HBsAg), the influenza A virus haemagglutinin, the herpes simplex virus (HSV) type 1 D glycoprotein, the rabies virus...

Topics

Animals
Antibodies, Viral
DNA, Recombinant
Genetic Vectors
Lethal Dose 50
Liver
Mice
Mice, Inbred BALB C
Mutation
Phenotype
Spleen
Thymidine Kinase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Vaccination

Vaccinia

Vaccinia virus

Viral Vaccines

Virulence

Virus Replication

Source

Open in PubMed

Identifiers

PMID: 4058585
DOI: 10.1038/317813a0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-4058585-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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