Insertional inactivation of the large subunit of ribonucleotide reductase encoded by vaccinia virus is associated with reduced virulence in vivo.

doi:10.1016/0042-6822(90)90119-c

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Article

Insertional inactivation of the large subunit of ribonucleotide reductase encoded by vaccinia virus is associated with reduced virulence in vivo.

Virology - 1 Feb 1990

Child S J, Palumbo G J, Buller R M, Hruby D E

Abstract excerpt

To assess whether a fully functional VV ribonucleotide reductase enzyme is required during both in vitro and in vivo replication of VV, three mutant viruses were constructed by marker transfer techniques: M1 lambda, an M1 insertion mutant; TK-, an insertion mutant of the VV thymidine kinase (tk)...

Topics

Animals
DNA Transposable Elements
Mice
Mice, Inbred BALB C
Mutation
Ribonucleotide Reductases
Thymidine Kinase
Vaccinia virus
Virulence
Virus Replication

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