Ultrafast Microdroplet Digestion of Antibodies with Fc-Silencing Mutations.

Abstract excerpt

Many novel therapeutic monoclonal antibody (mAb) modalities contain mutations that not only silence unwanted binding to Fc-gamma receptors but also could bring resistance to IdeS enzymatic cleavage into mAb subunits, limiting the antibody middle-down analysis by mass spectrometry (MS). Herein we showed, for the first time, the efficient, reproducible, and ultrafast microdroplet digestion (less than 1 ms) of mAbs...

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