A genome-wide screen identifies genes required for erythroid differentiation.

doi:10.1038/s41467-025-58739-w

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

A genome-wide screen identifies genes required for erythroid differentiation.

Nature communications - 12 Apr 2025

Myers Greggory, Friedman Ann, Yu Lei, Pourmandi Narges, Kerpet Claire, Ito Masaki A, Saba Rilie, Tang Vi, Ozel Ayse Bilge, Bergin Ingrid L, Johnson Craig N, Ku Chia-Jui, Wang Yu, Balbin-Cuesta Ginette, Lim Kim-Chew, Lin Zesen, Drysdale Claire, McGee Beth, Kurita Ryo, Nakamura Yukio, Liu Xiaofang, Siemieniak David, Singh Sharon A, Lyssiotis Costas A, Maillard Ivan, Weisman Lois S, Engel James Douglas, Khoriaty Rami

Abstract excerpt

The complete array of genes required for terminal erythroid differentiation remains unknown. To address this knowledge gap, we perform a genome-scale CRISPR knock-out screen in the human erythroid progenitor cell line HUDEP-2 and validate candidate regulators of erythroid differentiation in a custom secondary screen. Comparison of sgRNA abundance in the CRISPR library, proerythroblasts, and orthochromatic...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Differentiation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cell Line

Share to CRISPR-Cas Systems

Share to Erythroblasts

Share to Erythroid Cells

Share to Erythroid Precursor Cells

Share to Erythropoiesis

Share to Gene Knockout Techniques

Source

Open in PubMed Open full text

Identifiers

PMID: 40221460
DOI: 10.1038/s41467-025-58739-w
PMCID: PMC11993733

Genes

VAC14

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A genome-wide screen identifies genes required for erythroid differentiation.PMID 40221460Read ↗

A pooled CRISPR screen reveals genes critical for erythroblast enucleationSemantic proximity Read ↗

Putative regulators for the continuum of erythroid differentiation revealed by single-cell transcriptome of human BM and UCB cellsSemantic proximity Read ↗

RNA Editors Sculpt the Transcriptome During Terminal ErythropoiesisSemantic proximity Read ↗

Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide DonorsSemantic proximity Read ↗

RNA Editors Sculpt the Transcriptome During Terminal ErythropoiesisSemantic proximity Read ↗

Global discovery of erythroid long noncoding RNAs reveals novel regulators of red cell maturation.Semantic proximity Read ↗

Comprehensive Characterization and Global Transcriptome Analyses of Human Fetal Liver Terminal ErythropoiesisSemantic proximity Read ↗

Long noncoding RNA GATA2AS influences human erythropoiesis by transcription factor and chromatin landscape modulationSemantic proximity Read ↗

Epigenetic Regulation of Erythropoiesis: From Developmental Programs to Therapeutic TargetsSemantic proximity Read ↗

Blood Relatives: Splicing Mechanisms underlying Erythropoiesis in Health and DiseaseSemantic proximity Read ↗

Congenital dyserythropoietic anemiasSemantic proximity Read ↗

Global gene expression analysis of human erythroid progenitorsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.