Article
Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryo.
PLoS genetics - 1 Jan 2025
Formichetti Sara, Sadowska Agnieszka, Ascolani Michela, Hansen Julia, Ganter Kerstin, Lancrin Christophe, Humphreys Neil, Boulard Mathieu
Abstract excerpt
The reversible glycosylation of nuclear and cytoplasmic proteins (O-GlcNAcylation) is catalyzed by a single enzyme, namely O-GlcNAc transferase (OGT). The mammalian Ogt gene is X-linked, and it is essential for embryonic development and for the viability of proliferating cells. We perturbed OGT's function in vivo by creating a murine allelic series of four single amino acid substitutions, reducing OGT's catalytic...
Read the complete abstract on PubMedTopics
- Animals
- N-Acetylglucosaminyltransferases
- Mice
- Glycosylation
- Acetylglucosamine
- Embryonic Development