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Novel Method to Assess Macrophage Phenotype Using Eluted Media.

Cells, tissues, organs - 1 Jan 2025

Mahdi Furqan S, Lillyman David J, Ney Kayla E, Wachs Rebecca A

Abstract excerpt

INTRODUCTION: Macrophages exist on a spectrum from pro-inflammatory (M1) to pro-healing (M2). Characterization of macrophage phenotype is important to understand tissue healing and response. The gold standard for assessing macrophage phenotypes is immunocytochemistry (ICC), which stains inducible nitric oxide synthase (iNOS) and arginase (Arg1), the proteins secreted before nitrite and urea production. The ICC...

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Arginase
Arginine
Immunohistochemistry
Macrophages
Nitric Oxide Synthase Type II
Nitrites

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Rats, Sprague-Dawley

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PMID: 39701063
DOI: 10.1159/000543141

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