Article
Novel Markers to Delineate Murine M1 and M2 Macrophages.
PloS one - 1 Jan 2015
Jablonski Kyle A, Amici Stephanie A, Webb Lindsay M, Ruiz-Rosado Juan de Dios, Popovich Phillip G, Partida-Sanchez Santiago, Guerau-de-Arellano Mireia
Abstract excerpt
Classically (M1) and alternatively activated (M2) macrophages exhibit distinct phenotypes and functions. It has been difficult to dissect macrophage phenotypes in vivo, where a spectrum of macrophage phenotypes exists, and also in vitro, where low or non-selective M2 marker protein expression is observed. To provide a foundation for the complexity of in vivo macrophage phenotypes, we performed a comprehensive...
Topics
- Animals
- Biomarkers
- Flow Cytometry
- High-Throughput Nucleotide Sequencing
- Immunoenzyme Techniques
- Inflammation
- Lipopolysaccharides
- Macrophage Activation
- Macrophages
- Mice
- Mice, Inbred C57BL