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Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3

21 Nov 2024

Abstract excerpt

We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture efficiency and gene detection sensitivity. In a rigorous comparison with the 10X platform using human primary CD4 + T-cells, HT Smart-seq3...

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PMID: 39574011
DOI: 10.1186/s12864-024-11036-0

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