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Article

Truncating the C terminus of formate dehydrogenase leads to improved preference to nicotinamide cytosine dinucleotide.

Scientific reports - 20 Nov 2024

Guo Xiaojia, Wang Xueying, Hu Yinghan, Zhang Lingyun, Zhao Zongbao K

Abstract excerpt

Formate dehydrogenase (FDH) is widely applied in regeneration of redox cofactors. There are continuing interests to engineer FDH for improved catalytic activity and cofactor preference. In the crystal structure of FDH from Pseudomonas sp. 101 (pseFDH), the C terminus with 9 amino acid residues cannot be resolved. However, our earlier work showed mutations at C terminus led pseFDH variants to favor a non-natural...

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Identifiers

PMID: 39562703
DOI: 10.1038/s41598-024-79885-z
PMCID: PMC11576888

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