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Article

A Unique Insertion Loop Facilitates Tight NAD+ Binding in Nicotinoprotein: Insights from In Vitro Loop Engineering and In Silico Studies.

International journal of molecular sciences - 3 Mar 2026

Xue Houcheng, Yanase Takumi, Okuda-Shimazaki Junko, Kawai Haruka, Miura Daimei, Asano Ryutaro, Ikebukuro Kazunori, Sode Koji, Tsugawa Wakako

Abstract excerpt

Nicotinoproteins are a group of NAD+-dependent dehydrogenases that bind NAD+ tightly and catalyze reactions without using free NAD+. In this study, we investigated the role of the unique insertion loop in nicotinoproteins. Carveol dehydrogenase (CADh), a short-chain dehydrogenase/reductase (SDR) nicotinoprotein, and β-hydroxybutyrate dehydrogenase from Alcaligenes faecalis (AfBHBDh), a non-nicotinoprotein...

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PMID: 41828585
DOI: 10.3390/ijms27052367
PMCID: PMC12985038

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