Article
A library approach for the de novo high-throughput isolation of humanized VHH domains with favorable developability properties following camelid immunization
27 Sept 2023
Abstract excerpt
In this study, we generated a novel library approach for high throughput de novo identification of humanized single-domain antibodies following camelid immunization. To achieve this, VHH-derived complementarity-determining regions-3 (CDR3s) obtained from an immunized llama (Lama glama) were grafted onto humanized VHH backbones comprising moderately sequence-diversified CDR1 and CDR2 regions similar to natural...
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