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Article

High fidelity DNA ligation prevents single base insertions in the yeast genome.

Nature communications - 9 Oct 2024

Williams Jessica S, Lujan Scott A, Arana Mercedes E, Burkholder Adam B, Tumbale Percy P, Williams R Scott, Kunkel Thomas A

Abstract excerpt

Finalization of eukaryotic nuclear DNA replication relies on DNA ligase 1 (LIG1) to seal DNA nicks generated during Okazaki Fragment Maturation (OFM). Using a mutational reporter in Saccharomyces cerevisiae, we previously showed that mutation of the high-fidelity magnesium binding site of LIG1Cdc9 strongly increases the rate of single-base insertions. Here we show that this rate is increased across the nuclear...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 39379399
DOI: 10.1038/s41467-024-53063-1
PMCID: PMC11461686

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