Article
Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.
Nucleic acids research - 23 Sept 2024
Pal Arijit, Ghosh Dipannita, Thakur Pratyusha, Nagpal Priya, Irulappan Madhumathi, Maruthan Karthik, Mukherjee Sanket, Patil Nikita G, Dutta Tanmay, Veeraraghavan Balaji, Vivekanandan Perumal
Abstract excerpt
The genomic landscape associated with early adaptation to ciprofloxacin is poorly understood. Although the interplay between core metabolism and antimicrobial resistance is being increasingly recognized, mutations in metabolic genes and their biological role remain elusive. Here, we exposed Escherichia coli to increasing gradients of ciprofloxacin with intermittent transfer-bottlenecking and identified mutations...
Topics
- Ciprofloxacin
- Escherichia coli
- Drug Resistance, Bacterial
- Mutation
- Anti-Bacterial Agents
- Escherichia coli Proteins
- Adaptation, Physiological
- Gene Expression Regulation, Bacterial
- Regulatory Sequences, Nucleic Acid