Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cloning-independent markerless gene editing in Streptococcus sanguinis: novel insights in type IV pilus biology

16 Nov 2016

Abstract excerpt

Streptococcus sanguinis, a naturally competent opportunistic human pathogen, is a Gram-positive workhorse for genomics. It has recently emerged as a model for the study of type IV pili (Tfp)-exceptionally widespread and important prokaryotic filaments. To enhance genetic manipulation of Streptococcus sanguinis, we have developed a cloning-independent methodology, which uses a counterselectable marker and allows...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biochemical and Structural Characterization
Share to RNA and protein synthesis mechanisms
Share to Streptococcal Infections and Treatments

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Source

Identifiers

PMID: 27903891
DOI: 10.1093/nar/gkw1177

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 24 Oct 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cloning-independent markerless gene editing in Streptococcus sanguinis: novel insights in type IV pilus biologyPMID 27903891Read ↗

Genetic Manipulation of Group A Streptococcus-Gene Deletion by Allelic Replacement.Semantic proximity Read ↗

Genomic organization, structure, regulation and pathogenic role of pilus constituents in major pathogenic Streptococci and EnterococciSemantic proximity Read ↗

Streamlining marker-less allelic replacement in Streptococcus pneumoniae through a single transformation step strategy: easyJanus.Semantic proximity Read ↗

Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)Semantic proximity Read ↗

Adaptation of Prokaryotic Toxins for Negative Selection and Cloning-Independent Markerless Mutagenesis in Streptococcus Species.Semantic proximity Read ↗

Genome editing by natural genetic transformation in Streptococcus mutans.Semantic proximity Read ↗

Highly Effective Markerless Genetic Manipulation of Streptococcus suis Using a Mutated PheS-Based Counterselectable MarkerSemantic proximity Read ↗

LytF contributes to pilus extrusion during natural competence in Streptococcus sanguinis SK36Semantic proximity Read ↗

LytF contributes to pilus extrusion during natural competence in <i>Streptococcus sanguinis</i> SK36Semantic proximity Read ↗

Relevance of Pili in Pathogenic Streptococci Pathogenesis and Vaccine DevelopmentSemantic proximity Read ↗

In-Frame and Unmarked Gene Deletions in Burkholderia cenocepacia via an Allelic Exchange System Compatible with Gateway TechnologySemantic proximity Read ↗

Full structure/function analysis of all the pilin subunits in a type 4 pilus: a complex of minor pilins in <i>Streptococcus sanguinis</i> mediates binding to glycansSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.