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Article

A modified two primer approach to oligonucleotide-directed in vitro mutagenesis.

Biochimie - 1 Jan 2000

Sims P F, Minter S J, Stancombe R, Gent M E, Andrews J, Waring R B, Towner P, Davies R W

Abstract excerpt

Using oligonucleotide-directed mutagenesis, we are trying to define the features of the protein structure that are important for the DNA and c-AMP binding by CAP from E. coli, the enzymic activity and putative DNA binding of dihydrofolate reductase of L. casei, and the functionally important regions of the self-splicing RNA of the r-RNA intron of Tetrahymena thermophila. We have used a modification of the method...

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PMID: 3910112
DOI: 10.1016/s0300-9084(85)80177-2

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