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Article

A modified two primer approach to oligonucleotide-directed in vitro mutagenesis.

Biochimie - 1 Jan 2000

Sims P F, Minter S J, Stancombe R, Gent M E, Andrews J, Waring R B, Towner P, Davies R W

Abstract excerpt

Using oligonucleotide-directed mutagenesis, we are trying to define the features of the protein structure that are important for the DNA and c-AMP binding by CAP from E. coli, the enzymic activity and putative DNA binding of dihydrofolate reductase of L. casei, and the functionally important regi...

Topics

Animals
Base Sequence
Carrier Proteins
Cyclic AMP Receptor Protein
Escherichia coli
Genes
Genes, Bacterial
Mutagens
Mutation
Oligodeoxyribonucleotides
RNA Splicing
RNA, Ribosomal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Receptors, Cyclic AMP

Structure-Activity Relationship

Tetrahydrofolate Dehydrogenase

Tetrahymena

Source

Identifiers

PMID: 3910112
DOI: 10.1016/s0300-9084(85)80177-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-3910112-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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